Cannabis Cultivation and Science Podcast

Episode 150: Plants Under Pressure: How Stress Shapes Secondary Metabolites (w/ Dr. José Leme)


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Can stress actually improve flower quality? In this episode, I sit down with Dr. José Leme to dive into the science of plant stimuli and how growers can apply it in practice. We cover:

🌿 Mechanical cues — how super cropping and vibration trigger plant defense pathways.

💧 Water restriction — why moderate, timed drought can shift carbon toward secondary metabolites like terpenes and cannabinoids.

🧪 Jasmonates (MeJA) — when they increase trichome density, and what trade-offs you need to consider.

🔬 Trichome measurement pitfalls — why bracts are the gold standard for consistent data.

🌱 Propagation & trial design — the importance of clonal fidelity and standardized protocols when testing stress strategies.

Dr. Leme and I also highlight practical guardrails for growers: cultivar differences, timing, and the “sweet spot” between beneficial stress and damaging stress.

📄 Referenced Studies:

Water Deficit Stress and Secondary Metabolites

Jasmonates in Growth, Trichomes, and Cannabinoids

Metabolic Responses to Water Deficit

Trichome Sampling & Quantification Methods

Trigonelline as a Biomarker for Drought Stress (Preprint)

Propagation & Clonal Fidelity in Cannabis Research

Additional vibration study discussed in the episode.

🎧 Whether you’re a grower, consultant, or researcher, this conversation will give you a framework for using stress not as a risk—but as a precision tool to enhance plant quality.


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Cannabis Cultivation and Science PodcastBy Tad Hussey

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