the bioinformatics chat

#9 Michael Tessler and Christopher Mason on 16S amplicon vs shotgun sequencing

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Michael Tessler

and
Christopher Mason
join me to talk about their comparison of
16S amplicon sequencing and
shotgun sequencing for quantifying microbial diversity.

Links:

  • The 2017 Nature paper that we discuss: Large-scale differences in microbial biodiversity discovery between 16S amplicon and shotgun sequencing

  • Michael’s et al. 2016 paper that describes their original 16S study: A Global eDNA Comparison of Freshwater Bacterioplankton Assemblages Focusing on Large-River Floodplain Lakes of Brazil

  • The sequencing data for these studies is available from NCBI:

    PRJNA310230
    (16S),
    PRJNA389803
    (shotgun)

  • Michael’s website

  • Christopher’s lab website

  • The Integrative Biology & Medicine
    • conference, featuring the talk on Postmodern Synthesis by Eugene Koonin

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