Actinomycin D-based method as an easy approach has several advantages, for example, it does not require the construction and introduction of exogenous genes into cells. Although this method does not permit quantitative estimates of mRNA stability, it allows the easy analysis of mutant genes and thus may be useful for measuring the mRNA stability mechanism. Moreover, actinomycin D-based methods can determine the abundance of mRNA sequences following transcription inhibition, which allows the assessment of mRNA decay and stability.

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