Dr. Thomas Hartung is a Professor at Johns Hopkins University and University of Konstanz in Germany, as well as the Director of the Center for Alternatives to Animal Testing. Dr. Sushant Kamath is a Senior Manager of Risk Assessment at Abbott Healthcare. In this podcast, Drs. Hartung and Kamath give a high level overview of the history and evolution of artificial intelligence (AI), different models used in AI, and also case examples of the use of AI in toxicology. We also discussed updates on the regulation of AI use in healthcare and toxicology and its ethical aspects. Last, we discussed challenges faced with AI and strategies to improve AI use. This podcast is incredibly informative, with general and high-level information about AI in toxicology—the future of AI is bright!