Ribonucleoprotein (RNP) complexes are formed by the binding of RNA and RBPs in cells. RBP mediates key RNA based processes, such as alternative splicing, polyadenylation, subcellular localization, translation, and miRNA regulation. Mutations in these proteins can lead to a variety of human diseases and disorders. Techniques for testing RNA protein interaction have been developed from DNA protein interaction techniques with the same principles, and RIP is one of them.