Medizin - Open Access LMU - Teil 16/22

Carbon metabolism of enterobacterial human pathogens growing in epithelial colorectal adenocarcinoma (Caco-2) cells.


Listen Later

Analysis of the genome sequences of the major human bacterial pathogens has provided a large amount of information concerning their metabolic potential. However, our knowledge of the actual metabolic pathways and metabolite fluxes occurring in these pathogens under infection conditions is still limited. In this study, we analysed the intracellular carbon metabolism of enteroinvasive Escherichia coli (EIEC HN280 and EIEC 4608-58) and Salmonella enterica Serovar Typhimurium (Stm 14028) replicating in epithelial colorectal adenocarcinoma cells (Caco-2). To this aim, we supplied [U-(13)C(6)]glucose to Caco-2 cells infected with the bacterial strains or mutants thereof impaired in the uptake of glucose, mannose and/or glucose 6-phosphate. The (13)C-isotopologue patterns of protein-derived amino acids from the bacteria and the host cells were then determined by mass spectrometry. The data showed that EIEC HN280 growing in the cytosol of the host cells, as well as Stm 14028 replicating in the Salmonella-containing vacuole (SCV) utilised glucose, but not glucose 6-phosphate, other phosphorylated carbohydrates, gluconate or fatty acids as major carbon substrates. EIEC 4608-58 used C(3)-compound(s) in addition to glucose as carbon source. The labelling patterns reflected strain-dependent carbon flux via glycolysis and/or the Entner-Doudoroff pathway, the pentose phosphate pathway, the TCA cycle and anapleurotic reactions between PEP and oxaloacetate. Mutants of all three strains impaired in the uptake of glucose switched to C(3)-substrate(s) accompanied by an increased uptake of amino acids (and possibly also other anabolic monomers) from the host cell. Surprisingly, the metabolism of the host cells, as judged by the efficiency of (13)C-incorporation into host cell amino acids, was not significantly affected by the infection with either of these intracellular pathogens.
...more
View all episodesView all episodes
Download on the App Store

Medizin - Open Access LMU - Teil 16/22By Ludwig-Maximilians-Universität München


More shows like Medizin - Open Access LMU - Teil 16/22

View all
In search of dark matter by Prof. Dr. Andreas Burkert

In search of dark matter

0 Listeners

Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 03/06 by Ludwig-Maximilians-Universität München

Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 03/06

0 Listeners

Hegel lectures by Robert Brandom, LMU Munich by Robert Brandom, Axel Hutter

Hegel lectures by Robert Brandom, LMU Munich

6 Listeners

LMU Rechtsphilosophie by Prof. Dr. jur. Dr. jur. h.c. mult. Bernd Schünemann

LMU Rechtsphilosophie

0 Listeners

MCMP – Philosophy of Science by MCMP Team

MCMP – Philosophy of Science

1 Listeners

Epistemology and Philosophy of Science: Prof. Dr. Stephan Hartmann – SD by Ludwig-Maximilians-Universität München

Epistemology and Philosophy of Science: Prof. Dr. Stephan Hartmann – SD

2 Listeners

ISCB34 - 34th Annual Conference of the International Society for Clinical Biostatistics - Munich, 25-29 August 2013 by Prof. Dr. rer. nat. Ulrich Mansmann

ISCB34 - 34th Annual Conference of the International Society for Clinical Biostatistics - Munich, 25-29 August 2013

0 Listeners

MCMP – Philosophy of Physics by MCMP Team

MCMP – Philosophy of Physics

3 Listeners

Strings Conference 2012 (LMU) by Prof. Dr. Dieter Lüst et al.

Strings Conference 2012 (LMU)

0 Listeners

Women Thinkers in Antiquity and the Middle Ages - SD by Peter Adamson

Women Thinkers in Antiquity and the Middle Ages - SD

0 Listeners