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CCR5 | Investigating myself
Episode 373. Investigating myself. Topic: CCR5. How does HIV immunity work? Do I have deletions in my CCR5 gene? How can I find this out?
Twitter: @3minutelesson
Email: [email protected]
New episode every Monday, Wednesday, and Friday!
Methodology notes:
I searched the hg38 BAM file at chr3:46373450-46373488 (samtools view) for the deletion of GTCAGTATCAATTCTGGAAGAATTTCCAGACA. Reads contained the full sequence. I included a search for sequences with concatenated flanking nucleotides (indicating the deletion) but did not find any. I also searched unmapped reads. This strongly indicated that I am homozygous for the intact CCR5.
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By 3MLEpisode 373. Investigating myself. Topic: CCR5. How does HIV immunity work? Do I have deletions in my CCR5 gene? How can I find this out?
Twitter: @3minutelesson
Email: [email protected]
New episode every Monday, Wednesday, and Friday!
Methodology notes:
I searched the hg38 BAM file at chr3:46373450-46373488 (samtools view) for the deletion of GTCAGTATCAATTCTGGAAGAATTTCCAGACA. Reads contained the full sequence. I included a search for sequences with concatenated flanking nucleotides (indicating the deletion) but did not find any. I also searched unmapped reads. This strongly indicated that I am homozygous for the intact CCR5.