Physik - Open Access LMU - Teil 02/02

Cellular self-organization on micro-structured surfaces


Listen Later

Micro-patterned surfaces are frequently used in high-throughput single-cell studies, as they allow one to image isolated cells in defined geometries. Commonly, cells are seeded in excess onto the entire chip, and non-adherent cells are removed from the unpatterned sectors by rinsing. Here, we report on the phenomenon of cellular self-organization, which allows for autonomous positioning of cells on micro-patterned surfaces over time. We prepared substrates with a regular lattice of protein-coated adhesion sites surrounded by PLL-g-PEG passivated areas, and studied the time course of cell ordering. After seeding, cells randomly migrate over the passivated surface until they find and permanently attach to adhesion sites. Efficient cellular self-organization was observed for three commonly used cell lines (HuH7, A549, and MDA-MB-436), with occupancy levels typically reaching 40-60% after 3-5 h. The time required for sorting was found to increase with increasing distance between adhesion sites, and is well described by the time-to-capture in a random-search model. Our approach thus paves the way for automated filling of cell arrays, enabling high-throughput single-cell analysis of cell samples without losses.
...more
View all episodesView all episodes
Download on the App Store

Physik - Open Access LMU - Teil 02/02By Ludwig-Maximilians-Universität München


More shows like Physik - Open Access LMU - Teil 02/02

View all
Tonspur Forschung by Annik Rubens

Tonspur Forschung

3 Listeners

Einführung in die Ethnologie by Prof. Dr. Frank Heidemann

Einführung in die Ethnologie

0 Listeners

Theoretical Physics Schools (ASC) by The Arnold Sommerfeld Center for Theoretical Physics (ASC)

Theoretical Physics Schools (ASC)

2 Listeners

MCMP – Mathematical Philosophy (Archive 2011/12) by MCMP Team

MCMP – Mathematical Philosophy (Archive 2011/12)

6 Listeners

Hegel lectures by Robert Brandom, LMU Munich by Robert Brandom, Axel Hutter

Hegel lectures by Robert Brandom, LMU Munich

6 Listeners

MCMP – Metaphysics and Philosophy of Language by MCMP Team

MCMP – Metaphysics and Philosophy of Language

2 Listeners

MCMP – Philosophy of Science by MCMP Team

MCMP – Philosophy of Science

1 Listeners

Sommerfeld Lecture Series (ASC) by The Arnold Sommerfeld Center for Theoretical Physics (ASC)

Sommerfeld Lecture Series (ASC)

0 Listeners

MCMP by MCMP Team

MCMP

2 Listeners

Women Thinkers in Antiquity and the Middle Ages - SD by Peter Adamson

Women Thinkers in Antiquity and the Middle Ages - SD

0 Listeners