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Dynamic tracking and identification of tissue-specific secretory proteins in the circulation of live mice
Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2020.09.21.299198v1?rss=1
Authors: Kim, K.-e., Park, I., Kim, J., Kang, M.-G., Choi, W. G., Shin, H., Kim, J.-S., Rhee, H.-W., Suh, J. M.
Abstract:
Here we describe iSLET (in situ Secretory protein Labeling via ER-anchored TurboID) which labels secretory pathway proteins as they transit through the ER-lumen to enable dynamic tracking of tissue-specific secreted proteomes in vivo. We expressed iSLET in the mouse liver and demonstrated efficient in situ labeling of the liver-specific secreted proteome which could be tracked and identified within circulating blood plasma. iSLET is a versatile and powerful tool for studying spatiotemporal dynamics of secretory proteins, a valuable class of biomarkers and therapeutic targets.
Copy rights belong to original authors. Visit the link for more info
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By Multimodal LLCLink to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2020.09.21.299198v1?rss=1
Authors: Kim, K.-e., Park, I., Kim, J., Kang, M.-G., Choi, W. G., Shin, H., Kim, J.-S., Rhee, H.-W., Suh, J. M.
Abstract:
Here we describe iSLET (in situ Secretory protein Labeling via ER-anchored TurboID) which labels secretory pathway proteins as they transit through the ER-lumen to enable dynamic tracking of tissue-specific secreted proteomes in vivo. We expressed iSLET in the mouse liver and demonstrated efficient in situ labeling of the liver-specific secreted proteome which could be tracked and identified within circulating blood plasma. iSLET is a versatile and powerful tool for studying spatiotemporal dynamics of secretory proteins, a valuable class of biomarkers and therapeutic targets.
Copy rights belong to original authors. Visit the link for more info