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E3: SOLiD Sequencing
If ligation is more your thing
- Special Operations explaining SOLiD Sequencing: https://www.youtube.com/watch?v=YLT-DUeaLms
- Writeup by the Apollo Institute on SOLiD Sequencing: https://apollo-institute.org/solid-sequencing/
- Quick run-through video by ABI: https://www.youtube.com/watch?v=nlvyF8bFDwM
- Ars technica about the technology:https://arstechnica.com/science/2009/12/dna-sequencing-gets-solid-with-built-in-error-detection/
- atdbio explaining SOLiD sequencing:https://atdbio.com/nucleic-acids-book/Next-generation-sequencing#Sequencing-by-ligation-SOLiD
- Biogene Blog on SOLiD sequencers:https://www.creative-biogene.com/blog/index.php/2017/02/22/the-next-generation-sequencing-platform-of-abi-company/
Chapters:
00:00 Intro
02:45 Ligation
06:50 Getting hairy balls
20:00 Bead 1 with DNA polonies
23:30 6 Base Jokers
24:00 2-Base encoding colours
29:15 Offsets and holes
35:00 Randomness and barcodes
37:25 Chemistry summary
40:30 Homopolymers are not the problem
46:30 SOLiDs fate
Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany.
Listen to our podcast and we're happy to receive your feedback&questions via Email ( podcast[dot]explain[at]gmail[dot]com ).
The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).
Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).
License Notice
This podcast including all episodes, unless otherwise noted, is licensed under a Creative Commons Attribution 4.0 International License.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.
Attribution:
Podcast Title: Explain Podcast
Creators: Iuliia Novoselova, Daniel Rickert
Website: https://ngs-cn.de/explain-podcast/
View all episodes
By explain.podIf ligation is more your thing
- Special Operations explaining SOLiD Sequencing: https://www.youtube.com/watch?v=YLT-DUeaLms
- Writeup by the Apollo Institute on SOLiD Sequencing: https://apollo-institute.org/solid-sequencing/
- Quick run-through video by ABI: https://www.youtube.com/watch?v=nlvyF8bFDwM
- Ars technica about the technology:https://arstechnica.com/science/2009/12/dna-sequencing-gets-solid-with-built-in-error-detection/
- atdbio explaining SOLiD sequencing:https://atdbio.com/nucleic-acids-book/Next-generation-sequencing#Sequencing-by-ligation-SOLiD
- Biogene Blog on SOLiD sequencers:https://www.creative-biogene.com/blog/index.php/2017/02/22/the-next-generation-sequencing-platform-of-abi-company/
Chapters:
00:00 Intro
02:45 Ligation
06:50 Getting hairy balls
20:00 Bead 1 with DNA polonies
23:30 6 Base Jokers
24:00 2-Base encoding colours
29:15 Offsets and holes
35:00 Randomness and barcodes
37:25 Chemistry summary
40:30 Homopolymers are not the problem
46:30 SOLiDs fate
Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany.
Listen to our podcast and we're happy to receive your feedback&questions via Email ( podcast[dot]explain[at]gmail[dot]com ).
The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).
Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).
License Notice
This podcast including all episodes, unless otherwise noted, is licensed under a Creative Commons Attribution 4.0 International License.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.
Attribution:
Podcast Title: Explain Podcast
Creators: Iuliia Novoselova, Daniel Rickert
Website: https://ngs-cn.de/explain-podcast/