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E5: Illumina Sequencing Part I
Colour mixing in PHRED space
Chapters:
00:00 Nucleotides level 2
09:00 Cycles
12:00 Not-so-wobbly mutations
15:30 Reducing colourspace
22:30 Cutting connections
28:14 Costs and PHRED
- ClevaLabs on Illumina sequencing: https://www.youtube.com/watch?v=WKAUtJQ69n8
- Yourgenome on how the Illumina workflow works:https://www.yourgenome.org/facts/what-is-the-illumina-method-of-dna-sequencing/
- DNATech comparing Illumina Machines:https://dnatech.genomecenter.ucdavis.edu/illumina-high-throughput-sequencing/
- iBiology on sample preparation for Illumina sequencing:https://www.youtube.com/watch?v=PFwSe09dJX0
- StatQuest on how to use Illumina sequencing for RNA analysis:https://www.youtube.com/watch?v=tlf6wYJrwKY
- Illumina Flowcell and sequencing machines pictures:https://www.illumina.com/company/news-center/multimedia-images.html
- Nice explainer about paired-end sequencing:https://www.youtube.com/watch?v=gVrmfLXJRGM
Since 2018, West German Genome Center (https://wggc.de/)operates as the next generation sequencing facility and supportsresearch in genomics & transcriptomics by providing expertise andsequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/)- network of NGS core facilities in Germany.
Listen to our podcast and send your feedback to ( podcast[dot]explain[at]gmail[dot]com ).
The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).
Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).
CORRECTION: On the Illumina 1-channel chemistry, one base signal gets added, one removed at the enzymatic step: so that's one signal that stays, one that disappears, one that comes to shine and one that never shines. - DR
License Notice
This podcast including all episodes, unless otherwise noted, is licensed under a Creative Commons Attribution 4.0 International License.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.
Attribution:
Podcast Title: Explain Podcast
Creators: Iuliia Novoselova, Daniel Rickert
Website: https://ngs-cn.de/explain-podcast/
View all episodes
By explain.podColour mixing in PHRED space
Chapters:
00:00 Nucleotides level 2
09:00 Cycles
12:00 Not-so-wobbly mutations
15:30 Reducing colourspace
22:30 Cutting connections
28:14 Costs and PHRED
- ClevaLabs on Illumina sequencing: https://www.youtube.com/watch?v=WKAUtJQ69n8
- Yourgenome on how the Illumina workflow works:https://www.yourgenome.org/facts/what-is-the-illumina-method-of-dna-sequencing/
- DNATech comparing Illumina Machines:https://dnatech.genomecenter.ucdavis.edu/illumina-high-throughput-sequencing/
- iBiology on sample preparation for Illumina sequencing:https://www.youtube.com/watch?v=PFwSe09dJX0
- StatQuest on how to use Illumina sequencing for RNA analysis:https://www.youtube.com/watch?v=tlf6wYJrwKY
- Illumina Flowcell and sequencing machines pictures:https://www.illumina.com/company/news-center/multimedia-images.html
- Nice explainer about paired-end sequencing:https://www.youtube.com/watch?v=gVrmfLXJRGM
Since 2018, West German Genome Center (https://wggc.de/)operates as the next generation sequencing facility and supportsresearch in genomics & transcriptomics by providing expertise andsequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/)- network of NGS core facilities in Germany.
Listen to our podcast and send your feedback to ( podcast[dot]explain[at]gmail[dot]com ).
The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).
Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).
CORRECTION: On the Illumina 1-channel chemistry, one base signal gets added, one removed at the enzymatic step: so that's one signal that stays, one that disappears, one that comes to shine and one that never shines. - DR
License Notice
This podcast including all episodes, unless otherwise noted, is licensed under a Creative Commons Attribution 4.0 International License.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.
Attribution:
Podcast Title: Explain Podcast
Creators: Iuliia Novoselova, Daniel Rickert
Website: https://ngs-cn.de/explain-podcast/