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E7: PacBio
Zipping rounds in zeptoliters
Chapters:
00:00 Intro
07:00 Laser
09:00 Zeptoliters
13:30 Polymerase speed
19:00 Error rate
22:00 SMRT Cell
25:45 ccs
30:00 Fragment size
34:30 Megaruptor
38:20 Data flow
47:00 SVs
53:00 MAS Seq
- Starter: https://www.nature.com/articles/s41366-021-00824-3
- Karobben on PacBio sequencing: https://karobben.github.io/2023/10/30/Bioinfor/PacBio/
- bioinformticamente explaining the technology:https://bioinformaticamente.com/2020/12/05/pacbio-sequencing/
- Basic Biochem explainer of SMRT sequencing:https://www.youtube.com/watch?v=H2gUmRf-K1c
- Genomics Lab explaining it: https://www.youtube.com/watch?v=vRhUSjhygDU
- PacBio explaining BAM Tags: https://pacbiofileformats.readthedocs.io/en/13.0/BAM.html
- Megaruptor3 explainer (how we make the right sized fragments) :https://www.youtube.com/watch?v=zVhqnkQL8zo
- How we select the right sized fragments: https://www.youtube.com/watch?v=A25C8xfBbq8
Since 2018, West German Genome Center (https://wggc.de/)operates as the next generation sequencing facility and supportsresearch in genomics & transcriptomics by providing expertise andsequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/)- network of NGS core facilities in Germany. Listen to our podcast and we're happy to receive your feedback&questions via Email (podcast[dot]explain[at]gmail[dot]com ).
The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).
Our podcast is an outreach activity within the national genomicsfacility "West German Genome Center" funded by the DFG(https://www.dfg.de/) (No. 407493903).
License Notice
This podcast including all episodes, unless otherwise noted, is licensed under a Creative Commons Attribution 4.0 International License.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.
Attribution:
Podcast Title: Explain Podcast
Creators: Iuliia Novoselova, Daniel Rickert
Website: https://ngs-cn.de/explain-podcast/
View all episodes
By explain.podZipping rounds in zeptoliters
Chapters:
00:00 Intro
07:00 Laser
09:00 Zeptoliters
13:30 Polymerase speed
19:00 Error rate
22:00 SMRT Cell
25:45 ccs
30:00 Fragment size
34:30 Megaruptor
38:20 Data flow
47:00 SVs
53:00 MAS Seq
- Starter: https://www.nature.com/articles/s41366-021-00824-3
- Karobben on PacBio sequencing: https://karobben.github.io/2023/10/30/Bioinfor/PacBio/
- bioinformticamente explaining the technology:https://bioinformaticamente.com/2020/12/05/pacbio-sequencing/
- Basic Biochem explainer of SMRT sequencing:https://www.youtube.com/watch?v=H2gUmRf-K1c
- Genomics Lab explaining it: https://www.youtube.com/watch?v=vRhUSjhygDU
- PacBio explaining BAM Tags: https://pacbiofileformats.readthedocs.io/en/13.0/BAM.html
- Megaruptor3 explainer (how we make the right sized fragments) :https://www.youtube.com/watch?v=zVhqnkQL8zo
- How we select the right sized fragments: https://www.youtube.com/watch?v=A25C8xfBbq8
Since 2018, West German Genome Center (https://wggc.de/)operates as the next generation sequencing facility and supportsresearch in genomics & transcriptomics by providing expertise andsequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/)- network of NGS core facilities in Germany. Listen to our podcast and we're happy to receive your feedback&questions via Email (podcast[dot]explain[at]gmail[dot]com ).
The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).
Our podcast is an outreach activity within the national genomicsfacility "West German Genome Center" funded by the DFG(https://www.dfg.de/) (No. 407493903).
License Notice
This podcast including all episodes, unless otherwise noted, is licensed under a Creative Commons Attribution 4.0 International License.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.
Attribution:
Podcast Title: Explain Podcast
Creators: Iuliia Novoselova, Daniel Rickert
Website: https://ngs-cn.de/explain-podcast/