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E9: Single Cell Sequencing
Chapters:
00:00 Gambling
15:00 Bubbles
30:00 Holes
57:30 One more thing
1:07:00 Finale
1:08:00 Finale of season 1!
Eric Chow explaining single cell sequencing: https://www.youtube.com/watch?v=k9VFNLLQP8c
Overview of things to consider: https://www.youtube.com/watch?v=VWUPKrnRvoQ
How to analyze single cell data: https://www.youtube.com/watch?v=5HBzgsz8qyk
A very good single cell analysis toolkit: https://satijalab.org/seurat/
Explainer for the rough workflow:https://www.technologynetworks.com/genomics/articles/understanding-single-cell-sequencing-how-it-works-and-its-applications-357578
Quick explainer on single cell sequencing:https://www.the-scientist.com/single-cell-sequencing-in-a-nutshell-71048
A method-overview paper: https://www.nature.com/articles/s12276-020-00499-2
Comparing BD Parse and 10xGenomics: https://pubmed.ncbi.nlm.nih.gov/33414681/
Pseudotime analysis: https://bioconductor.org/books/3.14/OSCA.advanced/trajectory-analysis.html
Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany.
Listen to our podcast and send your feedback to ( podcast[dot]explain[at]gmail[dot]com ).
The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/). Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).
PS: I do not know how in the Parse approach the cells are treated to let the ligase and DNA enter each cell, but do not let RNA flow out of the cell at the same time. -DR
License Notice
This podcast including all episodes, unless otherwise noted, is licensed under a Creative Commons Attribution 4.0 International License.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.
Attribution:
Podcast Title: Explain Podcast
Creators: Iuliia Novoselova, Daniel Rickert
Website: https://ngs-cn.de/explain-podcast/
View all episodes
By explain.podChapters:
00:00 Gambling
15:00 Bubbles
30:00 Holes
57:30 One more thing
1:07:00 Finale
1:08:00 Finale of season 1!
Eric Chow explaining single cell sequencing: https://www.youtube.com/watch?v=k9VFNLLQP8c
Overview of things to consider: https://www.youtube.com/watch?v=VWUPKrnRvoQ
How to analyze single cell data: https://www.youtube.com/watch?v=5HBzgsz8qyk
A very good single cell analysis toolkit: https://satijalab.org/seurat/
Explainer for the rough workflow:https://www.technologynetworks.com/genomics/articles/understanding-single-cell-sequencing-how-it-works-and-its-applications-357578
Quick explainer on single cell sequencing:https://www.the-scientist.com/single-cell-sequencing-in-a-nutshell-71048
A method-overview paper: https://www.nature.com/articles/s12276-020-00499-2
Comparing BD Parse and 10xGenomics: https://pubmed.ncbi.nlm.nih.gov/33414681/
Pseudotime analysis: https://bioconductor.org/books/3.14/OSCA.advanced/trajectory-analysis.html
Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany.
Listen to our podcast and send your feedback to ( podcast[dot]explain[at]gmail[dot]com ).
The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/). Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).
PS: I do not know how in the Parse approach the cells are treated to let the ligase and DNA enter each cell, but do not let RNA flow out of the cell at the same time. -DR
License Notice
This podcast including all episodes, unless otherwise noted, is licensed under a Creative Commons Attribution 4.0 International License.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.
Attribution:
Podcast Title: Explain Podcast
Creators: Iuliia Novoselova, Daniel Rickert
Website: https://ngs-cn.de/explain-podcast/