The Single-Cell World Podcast

Ep15: Single-Cell DNA Sequencing Methods: an overview


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Hi there,

In this podcast episode, I will be talking about single-cell DNA. DNA is the information necessary to build our bodies. Alterations in DNA can cause changes in cell behavior, leading to diseases.

To study DNA at the single-cell level, we can use diferent protocols. Some examples are the whole-genome or the targeted approaches, the scATAC-seq or the scDNA methulation protocols.

Several commercial solutions are available and I will also mention them.

Listen now!
Cheers,
Cátia

Usefull Links
  • LAST DAY!! Single-Cell Course: Exploring the Cutting-Edge World of Single-Cell Technology (May 9, 2023-July 18, 2023

  • Support this Podcast


DNA ALTERATIONS KITS:

  • REPLI-g Advanced DNA Single Cell Kit (Qiagen)

  • GenomePlex® Single Cell Whole Genome Amplification Kit (Merck)

  • illustra™ Single Cell GenomiPhi™ DNA Amplification Kit (Merck)

  • Pico PLEX® Single Cell WGA Kit (Takara Bio)

  • AMPLI1 WGA PLUS KIT (Silicon biosystems)

  • MALBAC Single Cell WGA Kit (Yikon genomics)

  • ResolveDNA (Bioscryb Genomics)

  • scDNAseq kit (Mission Bio)


CHROMATIN ACESSIBILITY KITS:
  • ICELL8 scATAC-seq (Takara Bio)

  • scATAC-seq (10X Genomics)

  • SureCell ATAC-Seq Library Prep Kit (Biorad)


DNA METHYLATION KITS:
  • xGen™ Methylation-Sequencing DNA Library Preparation Kit (Integrated DNA Technologies)

  • TWIST NGS Methylation Detection System (Twist Bioscience)

  • scTAMseq(Mission Bio)



This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com
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The Single-Cell World PodcastBy Catia Moutinho