In this episode, the team discuss the omnipresent technology of next-generation sequencing (NGS), which has seen the cost of sequencing a human genome go from $100 million to under $1000 in just two decades. We hear from the European Bioinformatics Institute’s Dr Alex Almeida, who uses NGS techniques to examine the human microbiome. We also investigate the future of NGS and ask what will happen when we have sequenced absolutely everything?