The representation of macromolecular structures, for research or publication purposes, was a particular challenge for 20th century scientists. Focusing on protein science, this talk will explore how scientists and their collaborators have developed and used various techniques, from physical models to photorealistic computer graphics, to represent these structures. It will particularly focus on how the domains of science, the mechanical arts, the visual arts and computer science intersected and criss-crossed as this culture of macromolecular representation changed and evolved through the second half of the 20th century.