Organoids¬—miniaturized, three-dimensional, lab-grown, tissue cultures that mimic organs¬—can be used to improve drug discovery and safety testing by providing more predictive human biology models than traditional 2D cultures or animal testing. In this episode in our continuing series on discovery sciences with Charles River Laboratories, we explore the use organoids as powerful tools within the arsenal of New Approach Methodologies. We spoke to Charles River Labs Senior Research Leader Ludovico Buti, and Charles River Labs Chief Technology Officer for Discovery David Fischer, about the use of organoids, how Charles River is integrating these powerful tools into its work, and their potential to transform early discovery.