"Guitar Queer-O" is the thirteenth episode of the eleventh season and the 166th overall episode of the American animated television series South Park, which first aired on Comedy Central on November 7, 2007. The episode was rated TV-MA L in the United States. The episode parodies the Guitar Hero video game and aired ten days after the release of Guitar Hero III: Legends of Rock.

In the episode, Stan and Kyle are hooked on Guitar Hero, but Stan's superior skills in the video game damage his friendship with Kyle.