#25 — Phenol extraction of DNA is a commonly used method for removing proteins from nucleic acids; for example, to remove proteins from cell lysate during genomic DNA preparation.

It's commonly used, but not well understood.

Listen to this episode to get a quick explanation of how phenol extraction of DNA works.

Visit the original article to see handy diagrams on how phenol extraction works. [1] To get more help and advice on DNA extraction, read our related articles and discover how chloroform can clean up your phenol extractions, [2] learn how ethanol precipitation works, [3] and decide if ethanol or isopropanol is best for precipitating your samples. [4]

Resources:

1. The Basics: How Phenol Extraction of DNA Works. Available at: https://bitesizebio.com/384/the-basics-how-phenol-extraction-works/

2. Practical Application of Phenol-Chloroform Extraction. Available at: https://bitesizebio.com/3651/practical-application-of-phenol-chloroform-extraction/

3. Ethanol Precipitation of DNA and RNA: How it Works. Available at: https://bitesizebio.com/253/the-basics-how-ethanol-precipitation-of-dna-and-rna-works/

4. DNA Precipitation: Ethanol vs. Isopropanol. Available at: https://bitesizebio.com/2839/dna-precipitation-ethanol-vs-isopropanol/