In this episode, we dive into the heart of the cell—literally. Join us as we explore a groundbreaking study that maps the detailed in situ structure of the human prohibitin complex inside mitochondria. Using cutting-edge cryo-electron tomography, researchers have revealed a striking bell-shaped assembly formed by alternating PHB1 and PHB2 proteins. We discuss how this structure stabilizes mitochondrial membranes and forms specialized domains that could impact cell signaling, aging, and disease. With insights from structural biology, molecular modeling, and advanced microscopy, this episode unveils the dynamic yet ordered architecture of one of the most conserved protein families in the eukaryotic world.