Welcome all to IS PHARMACOLOGY DIFFICULT Podcast! I am Dr Radhika Vijay, in today's episode, I will be verbally putting up my thoughts about more details on my last topic about the second effector pathway of GPCR, namely Phospholipase C:IP3-DAG Pathway. As PKc phosphorylates  many intracellular proteins and leads to various physiological responses, it all serves signalling functions. Cytosolic calcium stays low in whole of this event, this is achieved by specific pumps located at plasma membrane and endoplasmic reticulum. Next, IP3 triggers release of Calcium, which serves as a third messenger! This calcium acts as highly versatile regulator through calmodulin (CAM) and PKc. Further many varied responses can be produced at a lot of  different sites. Lastly I will discuss most importantly the termination of the effector pathways , with the development of IP3 and DAG. Before I signature today's episode and crispy communications, a little short note about intracellular calcium release, a really short information that would maintain the zest of the session. Till the next episode, do follow me here, stay tuned, do rate and review on ITunes, Apple podcasts, thank you!!