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Jasmonic acid coordinates with light to regulate branching angle of Arabidopsis lateral roots
Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2020.08.11.245720v1?rss=1
Authors: Sharma, M., Sharma, M., Jamsheer K, M., Laxmi, A.
Abstract:
The role of jasmonates (JAs) in root growth and development and plants response to external stimuli is already known. However, its role in lateral root (LR) development remains perplexing. Our work identifies methyl jasmonate (MeJA) as a crucial phytohormone in determining the branching angle of Arabidopsis LRs. MeJA inclines the LRs to a more vertical orientation which was dependent on JAR1-COI1-MYC2,3,4 signalling. Our work highlights the dual role of light acting with MeJA in governing the LR angle. Glucose (Glc), produced by light mediated photosynthesis induces wider branching angles. Combining physiological and molecular assays, we reveal that Glc antagonizes the MeJA response via HEXOKINASE1 (HXK1) mediated signalling and by stabilizing JAZ9. Moreover, physiological assays using auxin mutants; binding of MYC2 on the promoters of auxin biosynthetic gene CYP79B2 and LAZY2 and asymmetric distribution of DR5::GFP and PIN2::GFP pinpoints the role of an intact auxin machinery required by MeJA for vertical growth of LRs. We also demonstrate that light perception through PHYTOCHROMES (PHYA and PHYB) and LONG HYPOCOTYL5 (HY5) are indispensable for inducing vertical angles by MeJA. Thus, our investigation highlights antagonism between light and Glc signalling and how they interact with JA-auxin signals to optimize the branching angle of LRs.
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By Multimodal LLCLink to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2020.08.11.245720v1?rss=1
Authors: Sharma, M., Sharma, M., Jamsheer K, M., Laxmi, A.
Abstract:
The role of jasmonates (JAs) in root growth and development and plants response to external stimuli is already known. However, its role in lateral root (LR) development remains perplexing. Our work identifies methyl jasmonate (MeJA) as a crucial phytohormone in determining the branching angle of Arabidopsis LRs. MeJA inclines the LRs to a more vertical orientation which was dependent on JAR1-COI1-MYC2,3,4 signalling. Our work highlights the dual role of light acting with MeJA in governing the LR angle. Glucose (Glc), produced by light mediated photosynthesis induces wider branching angles. Combining physiological and molecular assays, we reveal that Glc antagonizes the MeJA response via HEXOKINASE1 (HXK1) mediated signalling and by stabilizing JAZ9. Moreover, physiological assays using auxin mutants; binding of MYC2 on the promoters of auxin biosynthetic gene CYP79B2 and LAZY2 and asymmetric distribution of DR5::GFP and PIN2::GFP pinpoints the role of an intact auxin machinery required by MeJA for vertical growth of LRs. We also demonstrate that light perception through PHYTOCHROMES (PHYA and PHYB) and LONG HYPOCOTYL5 (HY5) are indispensable for inducing vertical angles by MeJA. Thus, our investigation highlights antagonism between light and Glc signalling and how they interact with JA-auxin signals to optimize the branching angle of LRs.
Copy rights belong to original authors. Visit the link for more info