GUEST: Clemens C.W.G. Lowik, Rotterdam
In this episode we discuss and learn about Bioluminescence Imaging and modern tools for multiplexed imaging of different colors, tackling a major challenge of the field. Our guest is one of the founding fathers of the ESMI and pioneers on multicolor BLI, the one and only  Clemens Lowik.
We talk about innovative approaches for unmixing multiple luciferase-luciferin pairs and quantitative analyses of bioluminescent mixtures, enabling serial tracking of heterogeneous cell populations. On the course of our discussion we learn on how such methods will help advance BLI to cover new exciting regimes, but also spill over to the wider optical imaging.
If you’re on to exciting developments of the field - listen carefully!

Selected Publication: "Multiplexed bioluminescence imaging with a substrate unmixing platform" published in Cell Chemical Biology by the authors C. K. Brennan, Z. Yao, A.A. Ionkina, C.M. Rathbun, B. Sathishkumar, J.A. Prescher (University of California, Irvine, USA).

Publication reference: C. K. Brennan, Z. Yao, A.A. Ionkina, C.M. Rathbun, B. Sathishkumar, J.A. Prescher. Multiplexed bioluminescence imaging with a substrate unmixing platform. Cell Chem Biol. 2022 Nov 17;29(11):1649-1660.e4. doi: 10.1016/j.chembiol.2022.10.004. Epub 2022 Oct 24 .
https://www.cell.com/cell-chemical-biology/fulltext/S2451-9456(22)00358-0

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https://e-smi.eu/
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