The Clark Family Story: Tracing the Cause of Hemophilia by Jeffrey T. Bradley Ann Arbor Skyline High School Health & Medicine Magnet In collaboration with the Centers for Disease Control and Prevention Jeffrey T. Bradley CDC Science Ambassador Program , Ann Arbor Skyline Health & Medicine Magnet, www.SkylineHealthMedicine.com This lesson emphasizes the relationship between DNA sequences, mutations in DNA and the change in the resulting protein structure and function. Hemophilia A will be used as a real life example of how a mutation in DNA results in a change in a protein’s structure and resulting ability to function. In order to complete these activities, the students should have prior knowledge in DNA structure and mutations, protein synthesis, Mendelian genetics and sex-linked traits. Learning Outcomes • The student will be able to relate a DNA sequences to the resulting protein structure in the factor VIII gene. • The student will be able to demonstrate the use of restriction enzymes in the identification of a specific DNA sequence and explain how this can be used to diagnosis someone has having the hemophilia factor VIII mutation. • The student will be able to demonstrate how a protein’s structure and function can change with a change in the factor VIII DNA sequence (mutation). • The student will be able to explain the genetics of hemophilia inheritance and affect on the blood clotting process. • The student will be able to draw conclusions about the risk of passing on the mutated gene.