The American Veterinary Medical Association (AVMA) provides guidelines for the use of carbon dioxide (CO2) for wildlife animal euthanasia. Here are some of the best practices for CO2 euthanasia of wildlife animals according to AVMA:

Use by trained personnel: Only trained personnel should administer CO2 euthanasia. Proper training should include instruction on the equipment used, the method of administration, and the signs of successful euthanasia.

Environment: The euthanasia should be carried out in a quiet and stress-free environment with minimal distractions. This can help reduce the animal's anxiety and improve the overall process.

Equipment maintenance: The equipment used for CO2 euthanasia should be well-maintained and regularly checked for leaks and proper functioning.

Animal preparation: Prior to euthanasia, animals should be properly restrained, calm, and given appropriate sedation if necessary.

CO2 delivery: The CO2 should be delivered in a gradual and controlled manner to minimize the animal's distress. The flow rate and concentration of CO2 should be appropriate for the size of the animal and the equipment being used.

Monitoring: Personnel should monitor the animal throughout the euthanasia process to ensure that it is unconscious and experiencing minimal distress. Signs of successful euthanasia include a lack of movement, slowed or stopped breathing, and dilated pupils.

Disposal: After euthanasia, the animal's body should be disposed of in a respectful and appropriate manner according to local laws and regulations.

It's important to note that these guidelines are not exhaustive and may vary depending on the specific circumstances and type of wildlife animal being euthanized. It's always recommended to consult with a wildlife veterinarian or animal welfare professional for guidance on best practices for CO2 euthanasia of wildlife animals.

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