Medizin - Open Access LMU - Teil 12/22

Pioglitazone inhibits growth of carcinoid cells and promotes TRAIL-induced apoptosis by induction of p21(waf1/cip1)


Listen Later

Background/Aims: We investigated the effect of the peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonist pioglitazone on growth and TRAIL-induced apoptosis in carcinoid cells. Methods: Carcinoid cells were incubated without and with pioglitazone. Effects on growth were examined by cell count and cell cycle analysis. p21(waf1/cip1) expression was determined by Western blotting. Cytotoxicity assay was performed by FACS analysis. Results: Pioglitazone suppressed the growth and induced apoptosis of carcinoid cells. Additionally, pioglitazone significantly enhanced carcinoid cell death induced by tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL). The enhancement of TRAIL-induced apoptosis was associated with an upregulation of cyclin-dependent kinase inhibitor p21(waf1/cip1) in pioglitazone-treated carcinoid cells. Importantly, overexpression of p21(waf1/cip1) in carcinoid cells by adenoviral gene transfer of p21 sensitized them to TRAIL-induced apoptosis. Conclusions: These results suggest that pioglitazone inhibits cell growth and sensitizes cells to TRAIL-induced apoptosis by induction of p21(waf1/cip1). Therefore, pioglitazone can be an effective therapeutic adjuvant for the treatment of carcinoid tumors. Copyright (C) 2001 S. Karger AG, Basel.
...more
View all episodesView all episodes
Download on the App Store

Medizin - Open Access LMU - Teil 12/22By Ludwig-Maximilians-Universität München


More shows like Medizin - Open Access LMU - Teil 12/22

View all
In search of dark matter by Prof. Dr. Andreas Burkert

In search of dark matter

0 Listeners

Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 03/06 by Ludwig-Maximilians-Universität München

Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 03/06

0 Listeners

Hegel lectures by Robert Brandom, LMU Munich by Robert Brandom, Axel Hutter

Hegel lectures by Robert Brandom, LMU Munich

6 Listeners

LMU Rechtsphilosophie by Prof. Dr. jur. Dr. jur. h.c. mult. Bernd Schünemann

LMU Rechtsphilosophie

0 Listeners

MCMP – Philosophy of Science by MCMP Team

MCMP – Philosophy of Science

1 Listeners

Epistemology and Philosophy of Science: Prof. Dr. Stephan Hartmann – SD by Ludwig-Maximilians-Universität München

Epistemology and Philosophy of Science: Prof. Dr. Stephan Hartmann – SD

2 Listeners

ISCB34 - 34th Annual Conference of the International Society for Clinical Biostatistics - Munich, 25-29 August 2013 by Prof. Dr. rer. nat. Ulrich Mansmann

ISCB34 - 34th Annual Conference of the International Society for Clinical Biostatistics - Munich, 25-29 August 2013

0 Listeners

MCMP – Philosophy of Physics by MCMP Team

MCMP – Philosophy of Physics

3 Listeners

Strings Conference 2012 (LMU) by Prof. Dr. Dieter Lüst et al.

Strings Conference 2012 (LMU)

0 Listeners

Women Thinkers in Antiquity and the Middle Ages - SD by Peter Adamson

Women Thinkers in Antiquity and the Middle Ages - SD

0 Listeners