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SARS-CoV-2 neutralization and serology testing of COVID-19 convalescent plasma from donors with non-severe disease
Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2020.08.07.242271v1?rss=1
Authors: Gniadek, T. J., Thiede, J. M., Matchett, W. E., Gress, A. R., Pape, K. A., Jenkins, M. K., Menachery, V. D., Langlois, R. A., Bold, T. D.
Abstract:
We determined the antigen binding activity of convalescent plasma units from 47 individuals with a history of non-severe COVID-19 using three clinical diagnostic serology assays (Beckman, DiaSorin, and Roche) with different SARS-CoV-2 targets. We compared these results with functional neutralization activity using a fluorescent reporter strain of SARS-CoV-2 in a microwell assay. This revealed positive correlations of varying strength (Spearman r = 0.37-0.52) between binding and neutralization. Donors age 48-75 had the highest neutralization activity. Units in the highest tertile of binding activity for each assay were enriched (75-82%) for those with the highest levels of neutralization.
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Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2020.08.07.242271v1?rss=1
Authors: Gniadek, T. J., Thiede, J. M., Matchett, W. E., Gress, A. R., Pape, K. A., Jenkins, M. K., Menachery, V. D., Langlois, R. A., Bold, T. D.
Abstract:
We determined the antigen binding activity of convalescent plasma units from 47 individuals with a history of non-severe COVID-19 using three clinical diagnostic serology assays (Beckman, DiaSorin, and Roche) with different SARS-CoV-2 targets. We compared these results with functional neutralization activity using a fluorescent reporter strain of SARS-CoV-2 in a microwell assay. This revealed positive correlations of varying strength (Spearman r = 0.37-0.52) between binding and neutralization. Donors age 48-75 had the highest neutralization activity. Units in the highest tertile of binding activity for each assay were enriched (75-82%) for those with the highest levels of neutralization.
Copy rights belong to original authors. Visit the link for more info