In this Tech Blast episode, we explore preparing 3D cell cultures with Sartorius’ (Goettingen, Germany) Sandra Söderholm, Application Development Specialist, and Maria Voutilainen, Product Manager. Sandra and Maria give us an introduction to 3D cell cultures and how to prepare them, and then delve into a foundational technique that is often overlooked: pipetting. We discuss pipetting techniques, common mistakes and their pipetting dos and don’ts.

Contents:

Introduction: 00:00–01:30

An introduction to 3D cell cultures: 01:30–02:48

Preparing 3D cell cultures: 02:48–03:25

Don’t overlook your pipetting technique: 03:25–05:45

Common pipetting mistakes and how to overcome them: 05:45–08:30

Reproducibility of 3D cell cultures: 08:30–10:30