In this Tech Blast episode, is Jay Lemke, Senior Research Scientist at Promega, provides a brief overview of Sanger sequencing, its history, working principle and development over the last half century.

Jay reveals which lab practices are still are best completed with Sanger sequencing and explains why the technique remains so prevalent today, even in the era of next-generation sequencing.

Get Jay's top tips for best practices when working with sanger and much more throughout the episode!

Contents:

 Introduction: 00:00-00:40

 A brief history of Sanger sequencing: 00:40-01:00

 Sanger sequencing working principle: 01:00-03:00

 How has sanger developed since it was invented? 03:00-05:10

 The advantages of Sanger in an era of NGS: 05:10-07:00

 Best applications of Sanger sequencing: 07:00-08:45

 Top tips for success with sanger: 09:30-10:10

 Challenges of working with sanger and addressing them: 10:10-11:35

 What would you improve about sanger sequencing? 11:35-12:25