TPX October 2015: Immunophenotype of Spontaneous Hematolymphoid Tumors Occurring in Young and Aging Female CD-1 Mice

This podcast with Dr. Jerold Rehg and hosted by Dr. Odete Mendes discusses the classification  of mice hematolymphoid tumors in CD1 mice, their pattern of incidence in regards to the age, sex or feeding status and their morphology, cell composition, organ involvement.  Immunohistochemical (IHC) markers that are diagnostically relevant  for these tumors were also discussed.  Dr Rehg characterized the cellular lineage of spontaneous hematolymphoid neoplasms arising in  female CD-1 mice.  Lymphoblastic lymphomas of T-cell and B-cell lineage were common in mice 12 months or less of age, whereas a wide range of non-lymphoblastic B-cell lymphomas and lymphoblastic T-cell lymphomas were common in mice older than 12 months. Additionally, he discussed the presence of renal hyaline droplets positive for lysozyme observed in aged mice with histiocytic-associated large B-cell lymphomas and myeloid leukemia. Finally, Dr Rehg also discussed the recovery of endogenous ecotropic MuLV genes from CD-1 mice and the expression of MuLV protein by IHC in lymphomas and some normal tissues of both young and aging CD-1 mice.  The data and methodology described by Dr Rehg will help differentiate and characterize spontaneous lymphomas and leukemias in CD-1 mice. The methodology described is also very relevant for broader immunophenotyping of genetically modified mice and drug immunomodulatory effects.

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