Between 1968 and 1970, American ethologist John B. Calhoun (1917-1995) conducted a behavioral study of captive mice within a nine-square-foot enclosure at a rural facility in Poolesville, Maryland. Within the enclosure known as Universe 25, several pairs of mice bred a population, which ultimately swelled to 2,200. Eventually, they established social orders that created inside and outside factions, and soon mating ceased altogether.