Link to bioRxiv paper:

http://biorxiv.org/cgi/content/short/2020.10.04.325548v1?rss=1

Authors: Zhang, H., De Silva, D., Dissanayaka, D., WARKENTIN, T. D., Vandenberg, A.

Abstract:

A validated method for B vitamin separation and quantification using ultra-performance liquid chromatography-selected reaction monitoring mass spectrometry (UPLC-SRM MS) was reported. For B vitamin extraction from lentil seeds, the use of three enzymes (acid phosphatase, {beta}-glucosidase, and rat serum) with a 4 h incubation was sufficient to convert bound B vitamins into their free forms. 20 B vitamers were selected and a 5-min UPLC-SRM MS method was optimized for rapid analysis. This method was applied to quantify B vitamin concentration during lentil seed germination. Total B vitamins increased up to 1.53-fold on day 5 (from 38.2 ug/g to 58.4 ug/g) comparing with dry seeds. Vitamin B5 (pantothenic acid) was the most abundant B vitamin in both dry seeds (32.7%) and in germinated seeds (16.5%-22.4% of total B vitamins), B8 (biotin) and B12 (cyanocobalamin) were not detected in lentil samples.

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