Physik - Open Access LMU - Teil 01/02

Velocity-Dependent Forces in Atomic Force Microscopy Imaging of Lipid Films


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We have imaged adsorbed fluid lipid bilayers by atomic force microscopy. The patches were formed by
rupture of phospholipid vesicles onto magnesium fluoride. We show that the membrane patches are fluid
but can be stably imaged at scan rates higher than 6 p d s . At lower scan rates the tip penetrates through
the layer. The penetrating tip does not destroy the fluid patches, and the previous image can be restored
after increasing the scanning velocity. The dynamic forces that possibly explain the effect are discussed.
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Physik - Open Access LMU - Teil 01/02By Ludwig-Maximilians-Universität München

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