Monitoring the release of neurotransmitters at spatial and temporal scales relevant to neuron function has always been a scientific challenge. This episode welcomes Dr. Beyene, who has developed an innovative solution to improve our understanding of chemical neurotransmission.

Dr. Beyene's groundbreaking research revolves around a chemi-sensitive, two-dimensional composite nanofilm that allows for the visualization of neurochemical dopamine release with unprecedented synaptic resolution and quantal sensitivity. This cutting-edge technology enables the observation of neurotransmitter release from hundreds of sites simultaneously, making it possible to monitor the spatiotemporal dynamics of dopamine release in dendritic processes—a phenomenon that has, until now, been poorly understood.

One of the most significant discoveries was that dopamine release is 'broadcast' from a subset of dendritic processes, referred to as 'hotspots.' These hotspots were found to co-localize with Bassoon, a protein known for organizing active zones in axon terminals. This suggests that Bassoon may also contribute to organizing active zones in dendrites.

Join us as we delve deep into the world of neurotransmission, exploring the intricacies of dopamine release, and discussing the possible implications of Dr. Beyene's findings for our understanding of neuronal communication.

Keywords: Neurotransmission, Dopamine, Composite Nanofilm, Synaptic Resolution, Dendritic Processes, Bassoon, Neuronal Communication.

Visualizing synaptic dopamine efflux with a 2D composite nanofilm https://doi.org/10.7554/eLife.78773