This comprehensive resource paper presents a multi-omic atlas of human bone marrow, combining single-cell RNA sequencing (scRNA-seq) and co-detection by indexing (CODEX) imaging to map cellular composition, spatial architecture, and cell-cell communication. The study reveals heterogeneity within mesenchymal stromal cells (MSCs), identifying distinct subsets like Adipo- and THY1+ MSCs as primary producers of hematopoietic support factors such as CXCL12, KITLG, CSF1, and IL-7. It further characterizes the spatial organization of hematopoietic stem and progenitor cells (HSPCs), showing a peri-adipocytic localization and an association of early myelopoiesis with a relatively oxygenated arterio-endosteal niche. Finally, the atlas is applied to acute myeloid leukemia (AML), demonstrating stromal expansion and altered cellular neighborhoods in disease, offering a valuable reference for future research into bone marrow niches in health and pathology.

References:

• Bandyopadhyay S, Duffy M P, Ahn K J, et al. Mapping the cellular biogeography of human bone marrow niches using single-cell transcriptomics and proteomic imaging[J]. Cell, 2024, 187(12): 3120-3140. e29.