This article detailis a comprehensive study on the splicing isoform landscape of the adult human heart under both normal conditions and in heart failure. Leveraging long-read single-nucleus RNA sequencing (snNanoRNAseq), the researchers generated an atlas of full-length RNA isoforms across ten major cardiac cell types. Key findings include that isoform heterogeneity is widespread in the heart and acts as a posttranscriptional buffer system; furthermore, they identified differential isoform usage (DIU) events that are specific to cell types and are significantly shifted in heart failure, particularly in cardiomyocytes. The study concludes that these cell-specific isoforms are crucial for understanding disease-associated cell states and offers a valuable resource for future mechanistic and translational studies, accessible via an online data portal.

References:

• Pan T, Lu L, Youker K, et al. Single-Cell Splicing Isoform Atlas of the Adult Human Heart and Heart Failure[J]. Circulation, 2025.

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