This paper details a systematic and in-depth analysis of GABAergic neurons within the mouse telencephalon, integrating high-resolution single-cell RNA sequencing (scRNA-seq) with spatial transcriptomics (MERFISH). The study establishes a comprehensive, hierarchical cell-type taxonomy for these inhibitory neurons, charting their complexity across various regions and developmental stages. A key finding is that the organization of these neurons reflects both their spatial location and their embryonic developmental origins from subpallial progenitor domains. Furthermore, the research highlights that related GABAergic cell types frequently undergo long-distance migration to populate disparate brain regions, and their diversification timing differs significantly between cortical/striatal neurons (postnatal) and septal/preoptic neurons (prenatal).

References:

• van Velthoven C T J, Gao Y, Kunst M, et al. Transcriptomic and spatial organization of telencephalic GABAergic neurons[J]. Nature, 2025, 647(8088): 143-156.