This research using spatial multi-omic technologies, such as deterministic barcoding in tissue (DBiT)-based spatial tri-omics (spatial ARP-seq and spatial CTRP-seq) and CODEX multiplexed imaging, to investigate the spatial dynamics of brain development and neuroinflammation. The study profiles the epigenome, transcriptome, and proteome simultaneously in mouse brains across various postnatal developmental stages and in a focal demyelination mouse model induced by lysolecithin (LPC), benchmarking findings against human brain development samples. Key results include the spatiotemporal regulation of gene expression and chromatin accessibility in cortical layers, the lateral-to-medial progression of myelination in the corpus callosum, and the delayed activation and distal spread of microglia/macrophages following the demyelinating lesion. The research integrates advanced sequencing and imaging techniques to offer a high-resolution compendium of molecular and cellular programs in the central nervous system.

References:

• Zhang D, Rubio Rodríguez-Kirby L A, Lin Y, et al. Spatial dynamics of brain development and neuroinflammation[J]. Nature, 2025, 647(8088): 213-227.