Nichoid-based 3D culture improves gene therapy by preserving hematopoietic stem cell (HSPC) functionality. This nanoengineered substrate regulates cytoskeletal architecture and metabolism to reduce DNA damage and oxidative stress, ensuring superior engraftment and clonal output.

References:

• Midena F, Alessandrini L, Conci C, et al. Nanoengineered 3D culture substrate enables superior persistence and polyclonal engraftment of genetically engineered hematopoietic stem cells[J]. Cell Stem Cell, 2026.