This research introduces DBiT-based spatial tri-omic technologies, specifically spatial ARP-seq and spatial CTRP-seq, to simultaneously map the epigenome, transcriptome, and proteome within the same tissue section. Researchers applied these tools to study mouse and human brain development, revealing how chromatin priming and gene expression coordinate the complex layering of the cerebral cortex. The study highlights a lateral-to-medial progression of myelination in the corpus callosum, steered by specific projection neuron tracts. Furthermore, the authors investigated neuroinflammation using a demyelination model, identifying distinct immune cell states and a delayed inflammatory response in distal white matter. By integrating multiple molecular layers, the sources provide a high-resolution atlas of the mechanisms governing central nervous system maturation and its reaction to injury.

References:

• Zhang D, Rubio Rodríguez-Kirby L A, Lin Y, et al. Spatial dynamics of brain development and neuroinflammation[J]. Nature, 2025, 647(8088): 213-227.