This study utilizes a multi-omic approach to map the spatial evolution and metastatic progression of high-grade serous ovarian cancer. By integrating whole-genome sequencing, single-cell transcriptomics, and spatial imaging, researchers developed the STARLETS framework to trace how hypoxia and immune pressure drive the selection of aggressive tumor clones. The findings highlight a unique tripartite ensemble consisting of specific macrophages, fibroblasts, and malignant cells that form spheroid structures to facilitate spreading within the abdominal cavity. Furthermore, the research identifies the SPP1-CD44 axis as a critical therapeutic target and demonstrates that SPP1+ macrophages can predict patient responses to oncolytic virus and PARP inhibitor treatments. Overall, the sources provide a comprehensive spatiotemporal panorama of how the tumor microenvironment shapes the trajectory of ovarian cancer metastasis.

References:

• Feng C, Yang Y, Li G, et al. Spatial tumor evolution panorama of ovarian cancer[J]. Cell Reports Medicine, 2026, 7(3).