The paper introduce SPTEdU-seq, a sophisticated spatial omics platform that simultaneously tracks newborn cell dynamics and maps the spatial total transcriptome. This innovative method utilizes optics-free detection via single-molecule probes and click chemistry, allowing researchers to study delicate cellular states without the damage caused by traditional imaging. By capturing both coding and non-coding RNAs as well as splicing isoforms, the technology provides a more comprehensive molecular view than previous techniques. The researchers demonstrated its utility by reconstructing regeneration trajectories in stroke models, tracing lineage maturation in embryos, and identifying heterogeneous splicing events in tumors. Ultimately, SPTEdU-seq offers a powerful framework for understanding complex cellular interactions and fate determinations within their native environments.

References:

• Niu H, Zhang S, Mao J, et al. SPTEdU-seq enables parallel optics-free newborn cell tracking and spatial total transcriptional dynamics in intact microenvironments[J]. Cell Stem Cell, 2026.