In this conversation, we explore the definitions and impact of unconscious and implicit biases on healthcare delivery and patient safety, centering specific biases including: relation, race, gender identity and expression, sexual orientation, age, and mental health. This discussion, an adjunct to the Whitepaper series on Recognizing and Managing Bias in Healthcare, facilitates evaluation of current healthcare environments and identification of strategies, tools, and interventions to address conscious or implicit bias in healthcare.