The mRNA isolation kit contains all necessary reagents and enough buffers for the isolation of mRNA from total RNA samples in less than one hour. mRNA isolation kits optimize the isolation of mRNA from different sample sizes or total RNA. The mRNA isolation kit allows for efficient and reproducible isolation of high-purity mRNA and permits the manual processing of multiple samples. It prepares large yields of highly purified mRNA from mammalian cells, tissue, and total RNA. The highly purified mRNA may be used directly in many molecular biology applications, such as RT-PCR, cDNA synthesis, northern blotting, northern ELISA, RNase protection assay, and even in vitro translation.