Examples: Maize, sugarcane, sorghum.
Adaptation: Found in hot, dry environments to minimize photorespiration.
Key Enzyme: PEP carboxylase, which has a high affinity for CO₂ and does not react with O₂, reducing photorespiration.
CO₂ + PEP (3C) → Oxaloacetate (4C) → Malate (4C).
Malate is transported to bundle-sheath cells, where CO₂ is released for the Calvin cycle.CAM (Crassulacean Acid Metabolism) Plants
Examples: Pineapple, cacti, succulents.
Adaptation: Found in arid environments; adapted to conserve water.
Stomata Behavior: Stomata open at night to minimize water loss.
Key Enzyme: PEP carboxylase, similar to C4 plants, used for initial carbon fixation at night.
CO₂ + PEP → Oxaloacetate (4C) → Malate (4C), which is stored in vacuoles as malic acid overnight.
During the day, malic acid is broken down to release CO₂ for the Calvin cycle.