The discussion of the prohibition of eating a forbidden item and whether it extends to benefiting as well- application to the rule of not placing a stumbling block before a blind person
The parameters of Rabbi Akiva's teaching on the impurity that gets passed on from an earthenware vessel- the principle of "tziruf" and its application to writing/erasing the letters before/after Hashem's name
The source of Rabbi Yosi's opinion in the teaching of his Rebbe- Rabbi Akiva- the unique law and deeper idea of earthenware that it can only become impure through what comes inside of it as opposed to its outer walls
The source and concept of a Biblical mikvah for vessels only requiring 3.5 ounces of water to purify- the debate and halacha of this kind of mikvah inside and outside the Beit Hamikdash