The study aimed to characterize the occurrence of tick-borne diseases within a herd in an endemic region in Brazil, focusing on two different aspects:

(a) to determine the occurrence of the three pathogens Anaplasma marginale, Babesia bovis and Babesia bigemina using direct and indirect detection methods and

(b) to analyze the incidence of genetic diversity among A. marginale isolates based on a Major Surface Protein (MSP) as a marker of variation within the farm.