Lentiviral vectors are gaining momentum not just as ex vivo tools but as potential in vivo therapeutic platforms. But with that shift comes a number of manufacturing challenges, including higher doses, tighter control of impurities, greater batch consistency, and scalable processes to meet both clinical and commercial needs.

In this GEN Podcast, two experts from SK Pharmteco, a global CMO, address these challenges and lay out some best practices that guide the manufacture of lentiviral vectors with the requisite purity, robustness, and economic feasibility required for widespread clinical adoption.

Guest speakers:

Mardhani Aparajithan, Director of Manufacturing, Science and Technology, SK Pharmteco

Tatiana Nanda, PhD, CTO, Cell and Gene Therapy, SK Pharmteco

Moderator:

Kevin Davies, PhD, Editorial Director, GEN

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Sponsored by Seqera

As data volumes in life sciences continue to grow, bioinformaticians and researchers are more dependent than ever on solutions that help them streamline workflows, manage data, and scale their research. In this timely podcast, Evan Floden, CEO and co-founder of Seqera, shares how his company is addressing these needs through Nextflow—the open-source orchestrator for scalable, reproducible, and portable scientific workflows—and Seqera Platform, a unified solution for the entire scientific data lifecycle. He also discusses how Seqera has become a widely adopted solution, trusted by 13 of the top 20 pharmaceutical companies, empowering breakthroughs in drug discovery, genomics, clinical research, and.beyond.

Hosted on Acast. See acast.com/privacy for more information.

Hosted on Acast. See acast.com/privacy for more information.

Hosted on Acast. See acast.com/privacy for more information.

In this episode, you’ll hear a group of experts discussing base editing and how the technology is helping scientists engineer powerful cell therapies for various diseases including novel CAR-T therapies and hypoimmunogenic induced pluripotent stem cells. During the discussion, they will cover the merits of the Pin-point base editing platform, which introduces gene modifications without relying on double-strand DNA breaks, as well as how TotalSeq reagents, which confirm the safety and efficiency of editing with the Pin-point platform, support single cell multiomics analysis of edited cells.

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This GENcast is the final installment in a 3-part series on Cas-CLOVER, “the proven alternative to CRISPR/Cas9,” sponsored by Demeetra AgBio. Demeetra has built extensive gene editing know-how, complementing its internally developed as well as globally sourced portfolio of intellectual property, which it transfers to its partners through simple commercial licenses with freedom to operate.

Jack Crawford, CEO of Demeetra, sat down with Dr. Leena Tripathi, Director of the Eastern Africa Hub and Leader of the Biotechnology Program at the International Institute of Tropical Agriculture (IITA), and David Norman, Senior Scientist in the Plant Systems Group at Demeetra.

They discuss how combining advanced gene editing techniques with traditional methods for plant breeding can meet the global challenges of feeding a rapidly growing population. Additionally, they discuss their brief communication in Plant Biotechnology Journal that was published in 2023 demonstrating Cas-CLOVER as an alternative genome-editing tool to CRISPR/Cas9 by targeting MusaPDS in the banana genome.

Hosted on Acast. See acast.com/privacy for more information.

This GENcast is part 2 of a 3-part series on Cas-CLOVER, “the proven alternative to CRISPR/Cas9,” sponsored by Demeetra AgBio. Demeetra has built extensive gene editing know-how, complementing its internally developed as well as globally sourced portfolio of intellectual property, which it transfers to its partners through simple commercial licenses with freedom to operate.

Jack Crawford, CEO of Demeetra, sat down with Dr. Molly Duman Scheel, professor in the department of medical and molecular genetics at the Indiana University School of Medicine - South Bend, and Dr. Corey Brizzee, director of gene editing at Demeetra.

The discussion focuses on the use of advanced gene editing technologies to create scalable, and eco-friendly biopesticides as described in the panelists’ latest publications in Journal of Fungi’s special issue “Development and Utilization of Yeast Resources” and Fermentation’s special issue “Advances in Yeast Biotechnology from Genomics to Industry.”

Panelists

“Two of the primary obstacles to using RNAi pesticides is the steep cost of production. How are you going to get it into the field, and how is it going to be stable, [gene edited] yeast solve both of these issues for us”—Dr. Molly Duman Scheel

“We wanted to make the expression as high as we could…we created a biomanufacturing platform [in yeast] with Cas-CLOVER by editing nutritional markers…this allowed for easy selection when we combined [the platform] with piggyBac to deliver the biopesticide RNAi”—Dr. Corey Brizzee

Hosted on Acast. See acast.com/privacy for more information.

Hosted on Acast. See acast.com/privacy for more information.