The built environment is made up of spatial prompts that provide overt and subtle messages to the occupants of the environment about its usage and appropriation. Design Factories which are both working and learning environments situated in universities and research institutions utilize spatial prompts both overtly and subtle to communicate, facilitate and enhance the culture of Design Factories - a culture of co-creation, experimentation and innovation. These spatial prompts are analogue and digital and they are both permanent and temporary. We will discuss the purposes behind the spatial prompts of Design Factories and how they have been specifically implemented to assist in the creation and continual development of a culture of co-creation, experimentation and innovation.