iBiology Podcast

In this talk, Dr. Anne Carpenter provides an overview of CellProfiler, a free, open-source software program for image analysis. CellProfiler helps scientists to identify and measure biological entities, process images, and export data for further analysis. Carpenter provides examples on how to use CellProfiler, and explains how CellProfiler can aid scientists in their bioimage analysis.

Modern microscopy produces large multi-dimensional datasets, which creates new challenges for data storage, processing and visualization. In this talk, Dr. Loic Royer uses a developing drosophila melanogaster embryo to explain how to solve some of the challenges produced by multi-dimensional microscopy datasets.

In order to understand an image of a biological sample and what it represents, one needs to understand its metadata. Metadata is the information behind the image that shows the experimental procedure, image acquisition settings, and the analysis performed on the data in order to obtain the represented image. Dr. Jason Swedlow explains what constitutes image metadata, and provides examples on how to catalog, organize, analyze, and share the metadata of biological images.

Microscopy is a key technology driving biological discovery. Nowadays, microscopy based scientific findings must be substantiated by quantitative image analysis. The discipline concerned with such quantification of biological microscopy images is called bioimage analysis. Dr. Christian Tischer walks us through the main concepts of a typical bioimage analysis workflow. He explains how to quantitatively interpret the content of microscopy images and how to automatically detect objects in images and derive object based measurements. He also emphasizes the importance of visual inspection and quality control of automated image analysis. Finally, he presents an overview of current bioimage analysis tools and communities.

How do we visualize biological samples? In this talk, Dr. Nico Stuurman provides an overview of the different tools, equipment, and software available to acquire an image of a biological sample using a light microscope, and the considerations one needs to take when using these tools. This lecture will allow scientists to understand the principles behind image acquisition in order to improve and optimize the analysis of their sample.

Manu Prakash always yearned to know the why and the how of things. As a boy in India, he spent endless hours playing outside with animals and making flammable artifacts in an abandoned lab in the basement of his home. Having the chance to explore his surroundings with open-ended curiosity, he learned to find the sublime in the mundane. Today, as a world-renowned researcher and inventor at Stanford University, he continues to be inspired by these childhood lessons, and is creating low-cost tools to empower people around the globe to go on their own journey of science and discovery.

Rebecca Calisi Rodríguez’s research on pigeons, like her life, is one of charting an original path. Her experiences as a Mexican-Italian-American woman, professor, artist and mother have provided her with fascinating and unusual perspectives to study the biology of parental behavior. And in so doing, she is redefining what it means to be a scientist.

There is ample evidence that race can be a major factor in health outcomes. But racial and ethnic minorities are underrepresented in clinical and biomedical research. In this series, Dr. Esteban Burchard talks about the consequences of this underrepresentation and what does it mean for equity in research and medicine. He also explains how race is used by your doctor to make critical decisions about your health, and discusses what happens if you don’t fit neatly in one racial box, and how does this impact the medical treatment. Finally, Burchard discusses how scientific breakthroughs often come when problems are approached from different perspectives, and the importance of improving diversity and inclusion in science.

There is ample evidence that race can be a major factor in health outcomes. But racial and ethnic minorities are underrepresented in clinical and biomedical research. In this series, Dr. Esteban Burchard talks about the consequences of this underrepresentation and what does it mean for equity in research and medicine. He also explains how race is used by your doctor to make critical decisions about your health, and discusses what happens if you don’t fit neatly in one racial box, and how does this impact the medical treatment. Finally, Burchard discusses how scientific breakthroughs often come when problems are approached from different perspectives, and the importance of improving diversity and inclusion in science.

There is ample evidence that race can be a major factor in health outcomes. But racial and ethnic minorities are underrepresented in clinical and biomedical research. In this series, Dr. Esteban Burchard talks about the consequences of this underrepresentation and what does it mean for equity in research and medicine. He also explains how race is used by your doctor to make critical decisions about your health, and discusses what happens if you don’t fit neatly in one racial box, and how does this impact the medical treatment. Finally, Burchard discusses how scientific breakthroughs often come when problems are approached from different perspectives, and the importance of improving diversity and inclusion in science.